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x sypro orange protein gel stain  (Thermo Fisher)


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    Thermo Fisher x sypro orange protein gel stain
    X Sypro Orange Protein Gel Stain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sypro+orange+protein+gel+stain/TRIS-HCL/pm34156250__es1c02514_si_001-78-7-13
    Average 99 stars, based on 1 article reviews
    x sypro orange protein gel stain - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Staining:

    Article Title: Discovery of a non‐nucleotide stimulator of interferon genes (STING) agonist with systemic antitumor effect
    Article Snippet: We obtained the human STING protein (R232, H232, 293Q, AQ) bearing cytosolic residues (140‐379) and the mouse STING (R231) containing the residues (139‐378) from Novoprotein. .. STING protein (5 μM), DW18343 (50 μM), and SYPRO Orange protein gel stain (5000X) (Cat. code: S6651, Invitrogen) were mixed, then added an equal volume of a reaction buffer (150 mM NaCl and 10 mM HEPES, pH 7.5) to reach 40 μL as a total volume. ..

    Article Title: Developing nanobodies as allosteric molecular chaperones of glucocerebrosidase function.
    Article Snippet: Thermal unfolding of GCasewas followedby thermal shift assays using SYPRO orange fluorescence as dye and a CFX connect real-time PCR system (Bio-Rad)48. .. 0.25mg/mL (4 μM) GCase was incubated with 20 μM of the different Nbs for 30min on ice and combined with 5× SYPRO Orange protein Gel Stain (Thermo Fischer Scientific), in Mc Ilvaine buffer pH 5.2 or pH 7.0 consisting of 0.1M citric acid and 0.2M dibasic sodium phosphate in a total volume of 25 μL. ..

    Article Title: A metal ion mediated functional dichotomy encodes plasticity during translation quality control
    Article Snippet: .. Thermal shift assay was performed with 10 μg of proteins in buffer composed of 50 mM HEPES (pH 7.5), 10 mM MgCl 2 and100 mM KCl was mixed with 5X SYPRO Orange Protein Gel Stain (Thermo Scientific) and then melting curve was measured in Bio-RAD CFX Opus realtime PCR system. .. Purified untagged Pfu AlaX-M protein was screened for initial crystallization at 4 °C with Crystal screen 1 and 2 (Hampton Research, Aliso Viejo, CA).

    Article Title: Site-Specific Immobilization Boosts the Performance of a Galectin-1 Biosensor.
    Article Snippet: .. Thermal shift assays were performed using 5 μM of protein in PBS pH 7.4, with final lactose concentrations ranging from 0 to 160 mM and 4x SYPRO Orange protein gel stain (Thermo Fisher Scientific Inc.) in a total reaction volume of 25 μl. .. Measurements were performed using a CFX Opus 96, Real-Time PCR System (Bio-Rad Laboratories, Inc., Hercules, CA).

    Article Title: Phosphorylation-mediated disassembly of C-terminal binding protein 2 tetramer impedes epigenetic silencing of pluripotency in mouse embryonic stem cells
    Article Snippet: .. A total of 2 μg of either wild-type or S164E recombinant Ctbp2 protein was mixed with 5x SYPRO Orange protein gel stain (Invitrogen), along with 100 μM NADH or 10 mM HEPES-OH [pH 7.5] buffer containing 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 and 1.8 mM KH 2 PO 4 [pH 7.4]. ..

    Article Title: Molecular basis for human respiratory syncytial virus transcriptional regulator NS1 interactions with MED25
    Article Snippet: Human respiratory syncytial virus (RSV, A2 strain) ATCC Cat. No. R-1540 Escherichia coli BL21(DE3) Novagen Cat. No.69450 Antibodies anti-MED1 Bethyl labs A300-793A-T anti-HA tag Epycypher 13-2010 anti-ATF3 CST 33593T Negative Control Rabbit IgG CUT&RUN Antibody Epycypher 13-0042 anti-FLAG Sigma Aldrich F7425 anti-tubulin Sigma Aldrich T8328 .. NS1 protein Chatterjee et al., 2017 N/A Mutant NS1 proteins This paper N/A MED25 ACID protein This paper N/A Mutant MED25 proteins This paper N/A NS1-MED25 ACID protein This paper N/A GST-VWA protein This paper N/A MBP-ATF3 protein This paper N/A ATF3bZIP protein This paper N/A SYPRO Orange Protein Gel Stain Thermo Fisher Scientific Cat. No. S6650 2-mercapthoethanol SigmaAldrich Cat. No. M3148 PBS SigmaAldrich Cat. No. D8537 .. Wizard® Plus SV Minipreps DNA Purification System Promega Cat. No. A1460 Wizard® Plus SV Gel and PCR Clean-Up System Promega Cat. No. A9282 Crystallization Screens Hampton Research https://hamptonresearch.com/cat-1.html RNAeasy Micro QIAGEN Cat. No. 74004 CUTANA ChIC/CUT&RUN kit Epycypher Cat:14-1048 CUTANA CUT&RUN library prep kit Epycypher Cat:14-1001

    Article Title: Developing nanobodies as allosteric molecular chaperones of glucocerebrosidase function
    Article Snippet: Thermal unfolding of GCase was followed by thermal shift assays using SYPRO orange fluorescence as dye and a CFX connect real-time PCR system (Bio-Rad) . .. 0.25 mg/mL (4 μM) GCase was incubated with 20 μM of the different Nbs for 30 min on ice and combined with 5× SYPRO Orange protein Gel Stain (Thermo Fischer Scientific), in Mc Ilvaine buffer pH 5.2 or pH 7.0 consisting of 0.1 M citric acid and 0.2 M dibasic sodium phosphate in a total volume of 25 μL. ..

    Article Title: The molecular basis for acetylhistidine synthesis by HisAT/NAT16.
    Article Snippet: .. SYPRO Orange protein gel stain (Thermo Fisher Scientific) was at 5X concentration. .. The plate was gradually heated from 10 to 95 °Cwhile the fluorescence from the dye was measured using a CFX384 Touch Real-Time PCR detection system (Bio-Rad) at the BiSS core facility.

    Incubation:

    Article Title: Developing nanobodies as allosteric molecular chaperones of glucocerebrosidase function.
    Article Snippet: Thermal unfolding of GCasewas followedby thermal shift assays using SYPRO orange fluorescence as dye and a CFX connect real-time PCR system (Bio-Rad)48. .. 0.25mg/mL (4 μM) GCase was incubated with 20 μM of the different Nbs for 30min on ice and combined with 5× SYPRO Orange protein Gel Stain (Thermo Fischer Scientific), in Mc Ilvaine buffer pH 5.2 or pH 7.0 consisting of 0.1M citric acid and 0.2M dibasic sodium phosphate in a total volume of 25 μL. ..

    Article Title: Developing nanobodies as allosteric molecular chaperones of glucocerebrosidase function
    Article Snippet: Thermal unfolding of GCase was followed by thermal shift assays using SYPRO orange fluorescence as dye and a CFX connect real-time PCR system (Bio-Rad) . .. 0.25 mg/mL (4 μM) GCase was incubated with 20 μM of the different Nbs for 30 min on ice and combined with 5× SYPRO Orange protein Gel Stain (Thermo Fischer Scientific), in Mc Ilvaine buffer pH 5.2 or pH 7.0 consisting of 0.1 M citric acid and 0.2 M dibasic sodium phosphate in a total volume of 25 μL. ..

    Thermal Shift Assay:

    Article Title: A metal ion mediated functional dichotomy encodes plasticity during translation quality control
    Article Snippet: .. Thermal shift assay was performed with 10 μg of proteins in buffer composed of 50 mM HEPES (pH 7.5), 10 mM MgCl 2 and100 mM KCl was mixed with 5X SYPRO Orange Protein Gel Stain (Thermo Scientific) and then melting curve was measured in Bio-RAD CFX Opus realtime PCR system. .. Purified untagged Pfu AlaX-M protein was screened for initial crystallization at 4 °C with Crystal screen 1 and 2 (Hampton Research, Aliso Viejo, CA).

    Polymerase Chain Reaction:

    Article Title: A metal ion mediated functional dichotomy encodes plasticity during translation quality control
    Article Snippet: .. Thermal shift assay was performed with 10 μg of proteins in buffer composed of 50 mM HEPES (pH 7.5), 10 mM MgCl 2 and100 mM KCl was mixed with 5X SYPRO Orange Protein Gel Stain (Thermo Scientific) and then melting curve was measured in Bio-RAD CFX Opus realtime PCR system. .. Purified untagged Pfu AlaX-M protein was screened for initial crystallization at 4 °C with Crystal screen 1 and 2 (Hampton Research, Aliso Viejo, CA).

    Recombinant:

    Article Title: Phosphorylation-mediated disassembly of C-terminal binding protein 2 tetramer impedes epigenetic silencing of pluripotency in mouse embryonic stem cells
    Article Snippet: .. A total of 2 μg of either wild-type or S164E recombinant Ctbp2 protein was mixed with 5x SYPRO Orange protein gel stain (Invitrogen), along with 100 μM NADH or 10 mM HEPES-OH [pH 7.5] buffer containing 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 and 1.8 mM KH 2 PO 4 [pH 7.4]. ..

    Mutagenesis:

    Article Title: Molecular basis for human respiratory syncytial virus transcriptional regulator NS1 interactions with MED25
    Article Snippet: Human respiratory syncytial virus (RSV, A2 strain) ATCC Cat. No. R-1540 Escherichia coli BL21(DE3) Novagen Cat. No.69450 Antibodies anti-MED1 Bethyl labs A300-793A-T anti-HA tag Epycypher 13-2010 anti-ATF3 CST 33593T Negative Control Rabbit IgG CUT&RUN Antibody Epycypher 13-0042 anti-FLAG Sigma Aldrich F7425 anti-tubulin Sigma Aldrich T8328 .. NS1 protein Chatterjee et al., 2017 N/A Mutant NS1 proteins This paper N/A MED25 ACID protein This paper N/A Mutant MED25 proteins This paper N/A NS1-MED25 ACID protein This paper N/A GST-VWA protein This paper N/A MBP-ATF3 protein This paper N/A ATF3bZIP protein This paper N/A SYPRO Orange Protein Gel Stain Thermo Fisher Scientific Cat. No. S6650 2-mercapthoethanol SigmaAldrich Cat. No. M3148 PBS SigmaAldrich Cat. No. D8537 .. Wizard® Plus SV Minipreps DNA Purification System Promega Cat. No. A1460 Wizard® Plus SV Gel and PCR Clean-Up System Promega Cat. No. A9282 Crystallization Screens Hampton Research https://hamptonresearch.com/cat-1.html RNAeasy Micro QIAGEN Cat. No. 74004 CUTANA ChIC/CUT&RUN kit Epycypher Cat:14-1048 CUTANA CUT&RUN library prep kit Epycypher Cat:14-1001

    Concentration Assay:

    Article Title: The molecular basis for acetylhistidine synthesis by HisAT/NAT16.
    Article Snippet: .. SYPRO Orange protein gel stain (Thermo Fisher Scientific) was at 5X concentration. .. The plate was gradually heated from 10 to 95 °Cwhile the fluorescence from the dye was measured using a CFX384 Touch Real-Time PCR detection system (Bio-Rad) at the BiSS core facility.



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    Image Search Results


    ArgD binds CphB in both LAH and PAT conditions. A , the relative levels of ArgD and CphB under PAT and LAH in WT and Δ cphB were determined by immunodetection. Whole-cell lysates were separated on SDS-PAGE and blotted onto a PVDF membrane, and the ArgD and CphB proteins were detected using specific antibodies. The Ponceau staining of the membrane is shown for the loading control. Repetitions of the experiment and their statistical analysis are presented in . B , coimmunopurification of f.ArgD with CphB was performed using the same amounts of PAT- or LAH-grown f.argD/ Δ argD cells. The eluates were separated by SDS-PAGE together with the input lysates, including 50% of the lysate from the PAT-grown cells (PAT 50 ). The SYPRO-stained gel was subsequently blotted to a PVDF membrane, which was probed with specific antibodies against CphB and the FLAG tag of ArgD. The control f.ArgD pull-down prepared from the Δ cphB cells is shown in Ref. . CphB, cyanophycinase; f.ArgD, FLAG-tagged variant of ArgD; LAH, light-activated heterotrophic; PAT, photoautotroph; PVDF, polyvinylidene fluoride.

    Journal: The Journal of Biological Chemistry

    Article Title: Cyanophycinase is required for heterotrophy in cyanobacteria

    doi: 10.1016/j.jbc.2025.110791

    Figure Lengend Snippet: ArgD binds CphB in both LAH and PAT conditions. A , the relative levels of ArgD and CphB under PAT and LAH in WT and Δ cphB were determined by immunodetection. Whole-cell lysates were separated on SDS-PAGE and blotted onto a PVDF membrane, and the ArgD and CphB proteins were detected using specific antibodies. The Ponceau staining of the membrane is shown for the loading control. Repetitions of the experiment and their statistical analysis are presented in . B , coimmunopurification of f.ArgD with CphB was performed using the same amounts of PAT- or LAH-grown f.argD/ Δ argD cells. The eluates were separated by SDS-PAGE together with the input lysates, including 50% of the lysate from the PAT-grown cells (PAT 50 ). The SYPRO-stained gel was subsequently blotted to a PVDF membrane, which was probed with specific antibodies against CphB and the FLAG tag of ArgD. The control f.ArgD pull-down prepared from the Δ cphB cells is shown in Ref. . CphB, cyanophycinase; f.ArgD, FLAG-tagged variant of ArgD; LAH, light-activated heterotrophic; PAT, photoautotroph; PVDF, polyvinylidene fluoride.

    Article Snippet: The proteins were separated, visualized with SYPRO orange protein dye (Lumiprobe ProteOrange, catalog no.: 40210) and transferred onto a polyvinylidene fluoride membrane (Sigma–Aldrich, Immobilon-P, catalog no.: IPVH00010) that was subsequently incubated with primary anti-CphB and anti-ArgD antibodies ( ).

    Techniques: Immunodetection, SDS Page, Membrane, Staining, Control, FLAG-tag, Variant Assay